Associations between Variation in X Chromosome Male Reproductive Genes and Sperm Competitive Ability in Drosophila melanogaster

Table 6

Primers used to amplify the desired region of each of the 11 candidate genes.

Gene name	Forward primer (F)	Reverse primer (R)	PCR product
CG15208	CGCGATATGATCGAACAGTG	TTGCAAAATGCGAATAGCTG	787 bp
CG15200	TCCTTCAAAGTCAATGCTGAGA	TTGCAGTGAAGCACAAAACA	785 bp
CG4349	CGACCCAGTTGCCTTTTAGA	AAACAATTGATCGGCCAAAA	852 bp
CG15035	GATGTGACACCCCTTCGATT	CACTTTACCAGATCGCACCA	839 bp
CG15035	GCAGCTGTACGGCGTGTAT	TGACCACTTTGTTTTGGAGAGA	850 bp
CG12470	GATCACGTTGCTTTGCTGAA	ACGAAGAAGAGCCGGGTAAT	749 bp
CG9806	TATTTCGGGAGAGCAGTCGT	TGATGGAGAAGGCCAATTTC	821 bp
CG1559	GCAGATGGACGGTTTGCTAT	TGTTTGCGTGATTTTCCTTG	972 bp
CG9156	TCCATGAAACACGAACTGGA	GTCTGGAGAATGGGACTGGA	709 bp
CG17450	AGAAGAAGCCACCGAGGACT	AAAGTGGCCTACTCCGATTG	823 bp
CG17450	ATATCGCATCAGCCATGTCA	CCGAGAGGTTGCTGAAAGAC	945 bp
CG32819	GCCAGGCTACCGTTTAATCA	GAATTTGGACTGGGTGTTGG	987 bp
CG32820	GGCCCACATGCTAATCAACT	ATGACATGGCTGATGCGATA	913 bp