Site-Specific Insertion Polymorphism of the MITE Alex-1 in the Genus Coffea Suggests Interspecific Gene Flow
Table 1
(a) Insertion polymorphism of the Alex-1 MITE at the g3 locus among a representative set of Coffea species and a close relative Psilanthus ebracteolatus.
Species analyzed
No. of individuals
+/+
−/−
+/−
Origin
C. arabica
3
3
0
0
E. Africa, Ethiopia
C. eugenioides
9
9
0
0
E. Africa Kenya
C. pseudozanguebariae
11
0
11
0
E. Africa Kenya
C. racemosa
11
0
11
0
E. Africa Tanzania
C. liberica var liberica
10
0
10
0
W. Africa RCI
C. stenophylla
10
0
10
0
W. Africa RCI
C. humilis
10
0
8
2
W. Africa RCI
C. canephora
71
53
8
10
W. and C. Africa
C. congensis
5
5
0
0
C. Africa RCA
C. liberica var. dewevrei
10
4
3
3
C. Africa RCA
C. liberica var. koto
3
3
0
0
C. Africa Cameroon
C. brevipes
10
10
0
0
C. Africa Cameroon
C. heterocalyx
1
0
1
0
C. Africa Cameroon
C. anthonyi
7
0
7
0
C. Africa Cameroon
C. sp N’Koumbala
10
8
0
2
C. Africa Cameroon
C. sp Mayombé
3
3
0
0
C. Africa Congo R.
C. kapakata
2
2
0
0
C. Africa Angola
C. myrtifolia
3
0
3
0
Mauritius
C. resinosa
1
0
1
0
Madagascar
C. tsirananae
1
0
1
0
Madagascar
C. lancifolia
1
0
1
0
Madagascar
C. perrieri
1
0
1
0
Madagascar
C. sakarahae
1
0
1
0
Madagascar
C. millotii
1
0
1
0
Madagascar
C. dolichophyla
1
0
1
0
Madagascar
C. heimii
1
0
1
0
Madagascar
C. bertrandii
1
0
1
0
Madagascar
C. humblotiana
1
0
1
0
Comoros
P. ebracteolatus
1
0
1
0
W. Africa RCI
Total
200
100
83
17
E. Africa: East Africa; W. Africa: West Africa; C. Africa: Central Africa. RCI: République de Côte d’Ivoire (Ivory Coast); RCA: République Centre Africaine (Central African Republic).
(b) Insertion polymorphism of the Alex-1 MITE at the g3 locus among a representative set of the diversity groups of the Coffea canephora species as defined by Gomez et al. [19].
Diversity group
No. of individuals
+/+
−/−
+/−
Origin
D
29
28
0
1* (DEA)
Guinea/RCI
A
2
1
0
1* (AE)
Cameroon/Congo
B
3
3
0
0
RCA
C
9
8
0
1* (CE)
Cameroon/Congo/RCA
E
16
1
8
7* (DE)
Congo/Cameroon/RCA
O
12
12
0
0
Uganda
Total
71
53
8
10
+/+ & −/−: Homozygote for presence and absence, respectively; +/−: Heterozygote. *: intraspecific hybrids.
