Unique Evolution of Symbiobacterium thermophilum Suggested from Gene Content and Orthologous Protein Sequence Comparisons

Figure 1

Phylogenetic relationships on the basis of gene content comparisons among 50 Clostridia and Bacillus subtilis. The ortholog cluster analysis (minimum cluster size, 2) among the 51 bacteria was performed using the MBGD [13]. This analysis produced the gene presence/absence data matrix (10,636 genes × 51 organisms), which was used to generate the distance matrix between all pairs of the 51 bacteria. On the basis of the distance matrix, a neighbor-joining tree was reconstructed using MEGA software version 4 [14]. The bootstrap was performed with 1000 replicates. The bar indicates a 200-gene difference.