Sequence Analysis of SSR-Flanking Regions Identifies Genome Affinities between Pasture Grass Fungal Endophyte Taxa

Table 2

Characteristics of EST-derived SSR loci used for phylogenetic analysis of endophyte isolates.

	EST-SSR locus
	NCESTA1AB04	NCESTA1FH03	NCESTA1GA07	NLESTA1GF09	NLESTA1NF04
Product size (bp)	241–257	217–252	181–206	364–385	242–265
Number of indels	5	7	3	6	2
Number of unique SSR array variants	2	13	11	1	19
Number of unique gene variants	14	13	13	11	15
Composition of products	coding + 5′-UTR	unknown	intron + unknown	coding + intron	unknown
Length of unit for phylogenetic analysis^1 (bp)	195	163	116	321	166
CDS or exon	151		46	238
UTR or intron	44		70	83
Number of informative characters^1	40	37	23	44	51
CDS or exon	30		10	30
UTR or intron	10		13	14
Percent informative characters^1	21	21	20	14	31
CDS or exon	20		22	13
UTR or intron	23		19	17
Number of trees resolved	3	1	4	2	2

¹Each indel was coded as a single multistate character.