Table of Contents
International Journal of Evolutionary Biology
Volume 2012, Article ID 329434, 9 pages
Research Article

DNA Barcoding and Molecular Phylogeny of Drosophila lini and Its Sibling Species

1Department of Entomology, South China Agricultural University, Guangzhou 510642, China
2Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan
3Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming 650091, China
4Evolutionary Genetics Laboratory, Department of Biology, Tokyo Metropolitan University, Tokyo 192-03, Japan
5The Hokkaido University Museum, Hokkaido University, Sapporo 060-0810, Japan

Received 15 July 2011; Accepted 27 October 2011

Academic Editor: Artyom Kopp

Copyright © 2012 Yi-Feng Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Drosophila lini and its two sibling species, D. ohnishii and D. ogumai, are hardly distinguishable from one another in morphology. These species are more or less reproductively isolated. The mitochondrial ND2 and COI-COII and the nuclear ITS1-ITS2 regions were sequenced to seek for the possibility of DNA barcoding and to reconstruct the phylogeny of them. The character-based approach for DNA barcoding detected some diagnostic nucleotides only for monophyletic D. ogumai, but no informative sites for the other two very closely species, D. lini and D. ohnishii, of which strains intermingled in the molecular phylogenetic trees. Thus, this study provides another case of limited applicability of DNA barcoding in species delineation, as in other cases of related Drosophila species. The molecular phylogenetic tree inferred from the concatenated sequences strongly supported the monophyly of the cluster of the three species, that is, the lini clade. We propose some hypotheses of evolutionary events in this clade.