Figure 5: NJ tree inferred from the concatenated sequences (2442 bp) of three regions, ND2 (926 bp), COI-COII (842 bp), and ITS1-ITS2 (674 bp). The time scale (in Mya) was given to the tree on the basis of an estimated time, 5.4 Mya, for the divergence between D. melanogaster and D. simulans [35] as a calibration point. MEGA 5.05 [29] was used for constructing the tree (bootstrap test: 1000 replications; model: Kimura 2-parameter; gaps: treated by pairwise deletion). Numbers in parentheses above branches indicate divergence times (Mya), and those below branches bootstrap percentages.