Whole-Genome Profiling of a Novel Mutagenesis Technique Using Proofreading-Deficient DNA Polymerase 𝜹

Table 3

Sequencing and mapping statistics.

Sample name	Number of mapped unique reads	% mapped reads	% genome covered* by unique reads	Average coverage by unique reads
BY2961	11,155,487	96.13	94.97	87.9×
EMS1	5,406,681	96.94	94.81	42.2×
EMS2	6,240,554	97.26	94.85	48.7×
EMS3	5,275,583	98.12	94.81	41.2×
EMS4	4,502,271	97.17	94.80	35.2×
EMS5	4,113,345	96.27	94.83	32.1×
Mutator 1	9,612,541	93.93	94.90	75.8×
Mutator 2	5,111,531	92.39	94.79	39.9×
Mutator 3	5,649,822	96.11	94.95	44.1×
Mutator 4	4,226,405	98.79	94.85	33.0×
Mutator 5	9,855,938	97.36	95.10	77.6×

*Coverage is defined as the percentage of bases in the genome that have at least 1 uniquely mapped read at that position.