Whole-Genome Profiling of a Novel Mutagenesis Technique Using Proofreading-Deficient DNA Polymerase 𝜹

Table 4

Mutations at protein level.

	Mutator		EMS
	𝑛	%	𝑛	%
Total mutations	28	100	201	100
Preserved amino acids	2	7.1	74	36.8
Amino acid changes	24	85.7	123	61.2
Stop	2	7.1	4	2.0
Changes in codon letter	28	100	201	100
1st	11	39.3	64	31.8
2nd	13	46.4	65	32.3
3rd	4	14.3	72	35.8
Impact of amino acid change	24	100	123	100
Conservative^a	4	16.7	57	46.3
Nonconservative	20	83.3	66	53.7
Stop and Gly/Pro codons	4	15.4	4	3.1
Stop	2	50.0	4	100.0
Gly/Pro	2	50.0	0	0.0

^aConservative and nonconservative amino acid substitutions were defined according to the BLOSUM62 matrix [25].