Table of Contents
International Journal of Evolutionary Biology
Volume 2012 (2012), Article ID 870935, 7 pages
http://dx.doi.org/10.1155/2012/870935
Research Article

Microsatellites Cross-Species Amplification across Some African Cichlids

1UMR 110, Cirad-Ifremer INTREPID, 34398 Montpellier, France
2INRA, UMR 1313 Génétique Animale et Biologie Intégrative, 78352 Jouy-en-Josas, France
3Department of Biology, Reed College, Portland, OR 97202, USA
4AgroParisTech, UMR 1313, Génétique Animale et Biologie Intégrative, 75231 Paris, France
5Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JT, UK

Received 31 January 2012; Accepted 26 March 2012

Academic Editor: Kristina M. Sefc

Copyright © 2012 Etienne Bezault et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The transfer of the genomic resources developed in the Nile tilapia, Oreochromis niloticus, to other Tilapiines sensu lato and African cichlid would provide new possibilities to study this amazing group from genetics, ecology, evolution, aquaculture, and conservation point of view. We tested the cross-species amplification of 32 O. niloticus microsatellite markers in a panel of 15 species from 5 different African cichlid tribes: Oreochromines (Oreochromis, Sarotherodon), Boreotilapiines (Tilapia), Chromidotilapines, Hemichromines, and Haplochromines. Amplification was successfully observed for 29 markers (91%), with a frequency of polymorphic (P95) loci per species around 70%. The mean number of alleles per locus and species was 3.2 but varied from 3.7 within Oreochromis species to 1.6 within the nontilapia species. The high level of cross-species amplification and polymorphism of the microsatellite markers tested in this study provides powerful tools for a wide range of molecular genetic studies within tilapia species as well as for other African cichlids.