Table 1: Results of prediction software to determine whether proteins are transmembrane, secretory, or GPI-anchored. Transmembrane proteins were determined using the TMHMM server on the CBS Prediction Server with a posterior probability >0.8 and no N-terminus prediction on a transmembrane protein. N-terminus signaling peptides were elucidated using TargetP on the CBS Prediction Server, with a specificity >0.7 indicating a high probability of a N-terminus signaling peptide. GPI-SOM was used to predict C-terminus cleavage and anchoring sites. If a protein was not transmembrane and has both N-terminus and C-terminus it was predicted to be a GPI-anchored protein. If it had only a N-terminus prediction, it was classified as secretory protein. If it did not fit any category it was classified as a cytosolic-like protein.

Daphnia CA isoform
criteria ->
Transmembrane
>0.8 and no N-terminus
N-Terminus
>0.7 specificity
C-Terminus
Most probable
Prediction

CA5NoYesYesGPI-anchored
CA6ANoYesNotSecretory
CA6BNoYesNotSecretory
CA6CNoYesNotSecretory
CA6DNoYesNotSecretory
CA6ENo0.178NotCytosolic-like
CA6FNoYesYesGPI-anchored
CA6GNoYesNotSecretory
CA6HNoYesNotSecretory
CA7ANoYesNotSecretory
CA7BNoYesNotSecretory
CA7CNoYesNotSecretory
CA7DNoYesNotSecretory
CA7ENoYesNotSecretory
CA7FNoYesNotSecretory
CA7GNoYesNotSecretory
CA7HNoYesYesGPI-anchored
CA7INoYesNotSecretory
CA7JNoYesNotSecretory
CA7KNoYesYesGPI-anchored
CA7LNoYesNotSecretory
CA7MNoYesNotSecretory
CA7NNoYesNotSecretory
CA7ONoYesYesGPI-anchored
CA7PNoYesNotSecretory
CA7QNo0.288NotCytosolic-like