Results of prediction software to determine whether proteins are transmembrane, secretory, or GPI-anchored. Transmembrane proteins were determined using the TMHMM server on the CBS Prediction Server with a posterior probability >0.8 and no N-terminus prediction on a transmembrane protein. N-terminus signaling peptides were elucidated using TargetP on the CBS Prediction Server, with a specificity >0.7 indicating a high probability of a N-terminus signaling peptide. GPI-SOM was used to predict C-terminus cleavage and anchoring sites. If a protein was not transmembrane and has both N-terminus and C-terminus it was predicted to be a GPI-anchored protein. If it had only a N-terminus prediction, it was classified as secretory protein. If it did not fit any category it was classified as a cytosolic-like protein.
|Daphnia CA isoform|
>0.8 and no N-terminus