Heterogeneous Expression of Chondroitin Sulfate Glycosaminoglycans and Versican Proteoglycan during Early Development of Rathke’s Pouch

Figure 2

Sagittal sections showing CS-GAG and versican localization in Rathke’s pouch at HH17. (a) Low magnification image of TC2 CS-GAG localization associated with Rathke’s pouch (asterisk denotes lumen). Strong reactivity is noted particularly along pouch roof underlying diencephalon (di). Note reduced staining along distal half of floor and at pouch tip (small arrowhead). (b) Phase contrast image of panel a shows prechordal plate (pp) following cranial flexure to Rathke’s pouch anterior to notochord (nt). (c) Higher magnification of TC2 CS-GAG in panel a showing location of prechordal plate (small arrow). in: infundibulum. (d) Phase contrast image of (c). (e) TC2 and (f) versican (vs) double label shows colocalization surrounding Rathke’s pouch. Note staining along distal pouch floor (large arrowheads). (g) Overlay of panels (e and f) highlights colocalization. Small arrowhead shows area of reduced TC2 and versican at tip of pouch. (h) Phase contrast image of panels (e–g). Note prechordal plate (small arrow). (i) d1C4 CS-GAG localization in Rathke’s pouch. Note strong staining along entire perimeter of pouch including floor (large arrowhead) with exception of the distal pouch tip (small arrowhead). (j) Phase contrast image of (i). ic: internal carotid artery rudiment. (k) Perlecan (pl) highlights basement membrane structure associated with Rathke’s pouch. Note lack of staining at pouch apex. Scale bar in (b) =100 μm (a and b); (d) =50 μm (c and d); (j) =100 μm (e–k).

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