Heterogeneous Expression of Chondroitin Sulfate Glycosaminoglycans and Versican Proteoglycan during Early Development of Rathke’s Pouch

Figure 3

CS-GAG and versican localization in sagittal sections of Rathke’s pouch at HH22. (a) Low magnification image of TC2 CS-GAG reveals Rathke’s pouch associated staining along the pouch roof (small arrow; asterisk, lumen of Rathke’s pouch). Note widespread reactivity with head mesenchyme and between neuroepithelial cells of diencephalon. (b) Phase contrast image of (a). in: infundibulum; oc: optic chiasm; nc: notochord. (c) Versican (vs) stains in a TC2-like pattern along the roof of Rathke’s pouch (small arrow), head mesenchyme and within the diencephalon. (d) Low magnification of d1C4 shows reactivity in a basement membrane-like pattern around Rathke’s pouch. Note staining encompassing entire tip of pouch (large arrow). (e) Double labeling of TC2 and versican shows colocalization associated with the roof of Rathke’s pouch (small arrow) Note that versican and TC2 stain ECM throughout area between pouch roof and ventral diencephalon. (f) Phase contrast image of (e). di: diencephalon. (g) Higher magnification of d1C4 antigen expression surrounding Rathke’s pouch, including pouch tip (large arrow). (h) Perlecan (pl) localization shows separate basement membrane structure associated with diencephalon and roof of Rathke’s pouch (small arrow). Scale bar in (b) =100 μm (a–d); (f) =50 μm (e–h).

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