Structural Stereochemistry of Androstene Hormones Determines Interactions with Human Androgen, Estrogen, and Glucocorticoid Receptors

Table 1

Relative androgenicity of androstene hormones.

Hormone	EC50 (nM)	Androgenicity	P value
Testosterone	0.35	1	1
Δ^5-Androstene-3β, 17β-diol	1.8	1/5	<0.001
Δ^5-Androstene-3β, 17α-diol	21	1/60	<0.001
Δ^5-Androstene-3β, 7β, 17β-triol	464	1/1326	<0.001

The EC₅₀ was calculated as the 50% activation point. The androgenicity is the testosterone EC₅₀ divided by the androstene hormone EC₅₀. This data does not normalize the cellular uptake of androstene hormones.