Synthesis and Biological Activity of Arylspiroborate Salts Derived from Caffeic Acid Phenethyl Ester

Figure 5

Typical chromatograms with detection at 270 nm (a) and 236 nm (b) obtained when stimulating HEK293 in the presence of 1 μM of sodium salt 4. Peaks are identified as follows: (A) 19-OH PGB₂ (internal standard), (B) PGB₂ (internal standard), (C) CAPE (3) from sodium salt 4, as well as the 5-LO products, (D) 6-trans LTB₄, (E) 6-trans-12-epi LTB₄, (F) LTB₄, and (G) 5-HETE. The inset in (b) shows the UV spectrum of peak C, which matches the CAPE (3) spectrum in Figure 4. All 5-LO products are checked against known standards (for retention time and UV spectral match).

(a)

(b)