In Vitro and Ex Vivo Evaluation of Smart Infra-Red Fluorescent Caspase-3 Probes for Molecular Imaging of Cardiovascular Apoptosis
Figure 6
Flow cytometry analysis of Annexin V, QCASP4.1, and QCASP5.1 fluorescence in HME cells. Cells were either untreated (control) or treated with staurosporine 1 μM for 6 h, in the absence or the presence of the caspase-3 inhibitor Z-Asp(OMe)-Gln-Met-Asp(OMe)-CH2F (100 μM). ** versus control; †, †† versus staurosporine alone. Only cells displaying either an FL1 or an FL4 intensity, corresponding respectively to annexin V or QCASP staining, ranging from almost 10² to 104 for both SSC and FSC parameters were included in the analysis gate.