Table of Contents
International Journal of Molecular Imaging
Volume 2011, Article ID 828151, 7 pages
http://dx.doi.org/10.1155/2011/828151
Research Article

Immunolymphoscintigraphy for Metastatic Sentinel Nodes: Test of a Model

1Clinic of Plastic Surgery, Breast Surgery and Burns Unit, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, Denmark
2The Finsen Laboratory, Rigshospitalet, 2100 Copenhagen, Denmark
3Diagnostic Product Development Division, Exiqon A/S, 2950 Vedbaek, Denmark
4Clinic of Clinical Physiology, Nuclear Medicine, and PET, Rigshospitalet, 2950 Copenhagen, Denmark
5Novo Nordisk A/S, 2880 Bagsvaerd, Denmark
6Institute of Molecular Pathology, University of Copenhagen, 1165 Copenhagen, Denmark
7Faculty of Medical Laboratory Science, Metropolitan University College, 1150 Copenhagen, Denmark
8Cluster for Molecular Imaging, Faculty of Health Sciences, University of Copenhagen, 1165 Copenhagen, Denmark
9Department of Surgery, Lunds University Hospital, 221 85 Lund, Sweden
10Department of Biomedical Sciences, Faculty of Health Sciences, University of Copenhagen, 1165 Copenhagen, Denmark

Received 30 November 2010; Accepted 1 March 2011

Academic Editor: Francesca Pons

Copyright © 2011 A. H. Chakera et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aim. To develop a method and obtain proof-of-principle for immunolymphoscintigraphy for identification of metastatic sentinel nodes. Methods. We selected one of four tumour-specific antibodies against human breast cancer and investigated (1), in immune-deficient (nude) mice with xenograft human breast cancer expressing the antigen if specific binding of the intratumorally injected, radioactively labelled, monoclonal antibody could be scintigraphically visualized, and (2) transportation to and retention in regional lymph nodes of the radioactively labelled antibody after subcutaneous injection in healthy rabbits. Results and Conclusion. Our paper suggests the theoretical possibility of a model of dual isotope immuno-lymphoscintigraphy for noninvasive, preoperative, malignant sentinel node imaging.