Table of Contents
International Journal of Molecular Imaging
Volume 2012 (2012), Article ID 250834, 8 pages
http://dx.doi.org/10.1155/2012/250834
Research Article

Evaluation of Norepinephrine Transporter Expression and Metaiodobenzylguanidine Avidity in Neuroblastoma: A Report from the Children's Oncology Group

1Department of Pediatrics, UCSF School of Medicine, 505 Parnassus Avenue, M696, San Francisco, CA 94143-0106, USA
2Department of Bioengineering and Therapeutic Sciences, UCSF Schools of Pharmacy and Medicine, San Francisco, CA 94143-0912, USA
3Department of Pediatrics, Children’s Hospital of Philadelphia, University of Pennsylvania, Philadelphia, PA 19104, USA
4Epidemiology and Biostatistics, UCSF School of Medicine, San Francisco, CA 94107, USA
5Department of Pathology, Perelman School of Medicine, Children’s Hospital of Philadelphia, University of Pennsylvania, Philadelphia, PA 19104, USA
6Department of Pediatrics, University of Michigan Medical School, Ann Arbor, MI 48109, USA
7Department of Biostatistics, University of Florida, Gainesville, FL 32611, USA
8Children's Oncology Group Statistics and Data Center, Arcadia, CA 91006-3776, USA
9Department of Pediatrics, Children's Hospital Boston and Dana-Farber Harvard Cancer Center, Boston, MA 02215-5450, USA
10Department of Pediatrics, Duke University School of Medicine, Durham, NC 27705, USA
11Department of Hematology/Oncology, Princess Margaret Hospital for Children, Perth, WA 6008, Australia

Received 3 August 2012; Accepted 23 August 2012

Academic Editor: Jun Hatazawa

Copyright © 2012 Steven G. DuBois et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. 123I-metaiodobenzylguanidine (MIBG) is used for the diagnostic evaluation of neuroblastoma. We evaluated the relationship between norepinephrine transporter (NET) expression and clinical MIBG uptake. Methods. Quantitative reverse transcription PCR ( ) and immunohistochemistry (IHC; ) were performed for neuroblastoma NET mRNA and protein expression and correlated with MIBG avidity on diagnostic scans. The correlation of NET expression with clinical features was also performed. Results. Median NET mRNA expression level for the 19 MIBG avid patients was 12.9% (range 1.6–73.7%) versus 5.9% (range 0.6–110.0%) for the 8 nonavid patients ( ). Median percent NET protein expression was 50% (range 0–100%) in MIBG avid patients compared to 10% (range 0–80%) in nonavid patients ( ). MYCN amplified tumors had lower NET protein expression compared to nonamplified tumors (10% versus 50%; ). Conclusions. NET protein expression in neuroblastoma correlates with MIBG avidity. MYCN amplified tumors have lower NET protein expression.