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Gene | NM no | Forward primer, 5′-3′ | Reverse primer, 5′-3′ | 5′-Flourophore | TaqMan probe, 5′-3′ | 3′-Quencher | Amplicon length (bp) |
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Humane |
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h-ITGAV | NM_002210 | GGGTCAAGATCAGTGAGAAATCTTTAC | ATTCCTGTAACATCATGCTATTGCTAG | FAM | AGGAACCTGGACCCCTTACCCCAACTTT | BHQ-1 | 139 |
h-ITGB3 | NM_000212 | CTCCTGTCCCTCATCCATAGC | CAGCCAAGAGGTAGAAGGTAAATAC | CY5 | ACAGCACACCAAGGCACAGGGC | BHQ-2 | 103 |
h-VEGFA | NM_001025366 | GTGTGAGTGGTTGACCTTCCTC | CCGTATATAAAACACTTTCTCTTTTCTCTG | HEX | CCTGGTCCTTCCCTTCCCTTCCCGA | BHQ-1 | 125 |
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Mice |
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m-ITGAV | NM_008402 | ACGTTACATAGCATAGTACCTCTTC | TACTGATGGTCTAAATTTGAACTGC | FAM | TGCCCTAACGTAACGTCGAAGAGAGAAACC | BHQ-1 | 121 |
m-ITGB3 | NM_016780 | TCAACATCCTAAGATGCCGAGAG | CTGCTTGTTCTACTACTGGTCAAC | CY5 | ACGGTATCAAGCCTGCGGTCCAGATTC | BHQ-2 | 104 |
m-VEGFA | NM_001025250 | GGGACCCCTTCGTCCTCTC | GTCTCCTGGGGACAGAATTAGTG | HEX | ATCACACAAGGTCCTCCTGGGCTGT | BHQ-1 | 100 |
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Housekeeping genes |
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h-HPRT | NM_000194 | AAGCCTAAGATGAGAGTTCAA | GCTCTACTAAGCAGATGGC | FAM | AACATCTGGAGTCCTATTGACATCGC | BHQ-1 | 108 |
h-UBC | NM_021009 | CTGGAAGATGGTCGTACC | GTCAGGGTCTTCACGAAG | HEX | CACCTCTGAGACGGAGCACC | BHQ-1 | 107 |
h-RPLP | NM_001002 | TGTGCAGCTGATCAAGAC | AGCACTTCAGGGTTGTAG | CY5 | CTGCCATTGTCGAACACCTGC | BHQ-2 | 141 |
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