Table of Contents
International Journal of Molecular Imaging
Volume 2013 (2013), Article ID 279872, 9 pages
http://dx.doi.org/10.1155/2013/279872
Research Article

-NOTA-CHSg and -CHSg Labeled Microspheres for Lung Perfusion and Liver Radiomicrospheres Therapy Planning

1Biomedical Engineering Department, Florida International University, 10555 West Flagler Street, EC 2614, Miami, FL 33174, USA
2Herbert Wertheim College of Medicine, Florida International University, 1240 SW 108 Avenue, Path, University Park, FL 33174, USA
3Mount Sinai Medical Center, 4300 Alton Road, Miami Beach, FL 33140, USA
4Jackson North Medical Center, 160 NW 170th Street, North Miami Beach, FL 33169, USA

Received 12 September 2013; Revised 11 November 2013; Accepted 18 November 2013

Academic Editor: Adriaan A. Lammertsma

Copyright © 2013 Alejandro Amor-Coarasa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Fast biodegradable (12 h < half-life < 48 h) radioactive labeled microspheres are needed for PET and SPECT lung perfusion and radiomicrosphere therapy planning. An emulsion method was used to create 30.1 ±4.8 μm size range microspheres with biodegradable Chitosan glycol (CHSg). Microspheres were characterized and labeled with or as an alternative to MAA in perfusion PET and SPECT studies. Surface decoration of CHSg microspheres with p-SCN-Bn-NOTA was performed to increase   in vivo stability. was labeled directly to the CHSg microspheres. Labeling yield and in vitro radiochemical stability were evaluated. In vitro CHSg microsphere degradation half-life was ~24 hours in porcine blood. Labeled microspheres were injected into Sprague Dawley rats and biodistribution was determined after 2 and 4 hours. Both -CHSg and -NOTA-CHSg were quickly allocated in the lungs after injection. -CHSg showed 91.6 ± 6.5% and 83.2 ± 4.1% of the decay corrected injected activity remaining in the lungs after 2 and 4 hours, respectively. For the obtained -NOTA-CHSg microspheres, lung allocation was very high with 98.9 ± 0.2% and 95.6 ± 0.9% after 2 and 4 hours, respectively. The addition of p-SCN-Bn-NOTA acts as a radioprotectant eliminating the released activity from the lungs to the bladder protecting the other organs.