Table of Contents
International Journal of Molecular Imaging
Volume 2016, Article ID 8434308, 9 pages
http://dx.doi.org/10.1155/2016/8434308
Research Article

Visualization of Inflammation at Early Stage of Lung Cancer in Xenografted Temporally Immunosuppression Rats by Ferrioxamine Magnetic Resonance Imaging

1Laboratory of Physical Chemistry, Molecular, and Cellular Biology, Center of Excellence for Molecular Imaging, Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, 50200, Thailand
2Clinical Investigation Center of Besançon, Inserm CICB 1431, University Hospital of Besançon, 2, Place St. Jacques, 25030 Besançon, France
3Cutaneous Engineering and Biology Team, Inserm UMR 1098, University of Bourgogne Franche-Comté, 19 rue Ambroise Paré, Besançon, France
4Department of Structural Biology and Bioenergetics Mechanisms, Commission for Atomic Energy, Institute of Biology and Technologies-Saclay, 91191 Gif-sur-Yvette, France

Received 17 May 2016; Accepted 26 September 2016

Academic Editor: J. M. Mountz

Copyright © 2016 Nathupakorn Dechsupa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Physiological responses such as chronic inflammation and angiogenesis could be used as biomarkers for early detection of cancer with noninvasive imaging modalities. The present study reports the application of magnetic resonance imaging instrument to image the binding of ferrioxamine with hemin that allows visualizing the chronic inflammation foci of lung tissue of immunocompromised rats xenografted using small cell lung carcinoma. A low concentration of ferrioxamine (μM·kg−1 of rat weight) deposited on tissue outside the vasculature was found to diffuse across the capillary walls to the interstitial space and inflammation foci, which provided a clear enhancement of T1-weighted gradient-echo sequence images. Ferrioxamine imaging allowed the determination of inflammatory sites and their localization in 3D fat-suppressed maximum intensity projections. The smallest dimension of foci that can be clearly determined is about 0.1 mm3. In concomitant to the in vivo imaging, analysis of histological tissue section showed the development of inflammatory sites. This study provides evidence that medical imaging instrument such as MRI scanner allows researchers to correlate images taken with MRI with those using high-resolution microscopy. Moreover, ferrioxamine is a useful molecular probe for determining chronic inflammation particularly at the very early stages of cancer.