Table of Contents
Indian Journal of Materials Science
Volume 2014 (2014), Article ID 502429, 5 pages
http://dx.doi.org/10.1155/2014/502429
Research Article

Studies on Adsorption of DNA on Functional Ultrathin Films of Cationic Surfactant

Department of Physics, Birla Institute of Technology and Science, Pilani, Rajasthan 333031, India

Received 30 November 2013; Accepted 17 December 2013; Published 12 January 2014

Academic Editors: Koushik Dhara and Subrata Kundu

Copyright © 2014 Monika Poonia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We have investigated the interaction of DNA with a highly ordered functional ultrathin layer of cationic surfactant, dioctadecyl ammonium bromide (DOAB). The ultrathin film of DOAB is fabricated by Langmuir-Blodgett technique onto the pretreated quartz crystal wafers. The solution of DNA in phosphate buffer saline (PBS) is injected through a flow cell in a quartz crystal microbalance (QCM) loaded with the functional ultrathin film. The QCM data indicate a slower kinetics (time constant  =  162.2 seconds) for the adsorption of DNA on DOAB layer as compared to PBS on DOAB. The surface morphology of the aggregation of DNA over the DOAB layer is investigated using atomic force microscope (AFM). The AFM image indicates the trapping of DNA over the DOAB layer. Such trapping of DNA can be potentially employed in the field of genomics.