Table of Contents
International Journal of Peptides
Volume 2009 (2009), Article ID 362482, 4 pages
http://dx.doi.org/10.1155/2009/362482
Research Article

Effect of Microwave Radiation on Enzymatic and Chemical Peptide Bond Synthesis on Solid Phase

1Laboratory of Applied and Computational Biocatalysis, Department of Pharmaceutical Sciences, University of Trieste, Piazzale Europa 1, 34127 Trieste, Italy
2School of Chemistry, The University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
3Manchester Interdisciplinary Biocentre (MIB), The University of Manchester, 131 Princess Street, Manchester M1 7DN, UK

Received 15 October 2008; Accepted 4 February 2009

Academic Editor: Ayman El-Faham

Copyright © 2009 Alessandra Basso et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Peptide bond synthesis was performed on PEGA beads under microwave radiations. Classical chemical coupling as well as thermolysin catalyzed synthesis was studied, and the effect of microwave radiations on reaction kinetics, beads' integrity, and enzyme activity was assessed. Results demonstrate that microwave radiations can be profitably exploited to improve reaction kinetics in solid phase peptide synthesis when both chemical and biocatalytic strategies are used.