International Journal of Peptides / 2010 / Article / Tab 3

Review Article

Effect of Ghrelin on Glucose-Insulin Homeostasis: Therapeutic Implications

Table 3

Results obtained with cellular cultures and pancreatic perfusion that contribute to data about ghrelin role on glucose and insulin metabolism.

Cellular type/PerfusionTreatmentDoseInsulin releaseGlucose outputReference

Islets from normal ratsAG + glucose versus glucose10-12 M AG + 2.8 mM glucoseNot change [54]
AG + glucose versus glucose10-12 M AG + 8.3 mM glucoseEnhanced

Islets from normal ratsAG + glucose versus glucose10-8 M AG + 2.8 mM glucoseNot change [58]
AG + glucose versus glucose10-8 M AG + 8.3 mM glucoseDiminished

Islets from normal ratsAG + glucose versus glucose10 nM AG + 20 mM glucoseDiminished [92]
UAG + glucose versus glucose1  𝜇 M + 20 mM glucoseNot change
AG + glucose + YIL-781 versus glucose10 nM AG + 20 mM glucose + 1  𝜇 M YIL-781Not change
Glucose + YIL-781 versus glucose20 mM glucose + 1  𝜇 M YIL-781Not change

Islets from normal ratsGHRP-6 versus placebo1  𝜇 M GHRP-6Enhanced [58]
SPA versus placebo1  𝜇 M SPAEnhanced

Ghrelin KO mouse isletsGlucose ghrelin KO versus glucose wildtype8.3 mM and 16.7 mM glucoseEnhanced [91]

Min 6 cellsAG + glucose versus glucose1–10 nM AG + 22.2 mM glucoseDiminished [93]

Hepatocytes from pigsAG versus placebo100 nM AGEnhanced [96]
UAG versus placebo100 nM UAGDiminished
UAG + AG versus AG100 nM AG + 100 nM UAGDiminished

Pancreas of rat perfused in situGhrelin + glucose versus glucose10 nM ghrelin + 5.5 mM glucoseNot change [90]
Ghrelin + glucose versus glucose10 nM ghrelin + 9 mM glucoseDiminished

Pancreas of rat perfused in vitroGhrelin + glucose versus glucose10 nM ghrelin + 8,3 mM glucoseDiminished [91]
GHRP-6 + glucose versus glucose1  𝜇 M GHRP-6 + 8.3 mM glucoseEnhanced
UAG + glucose versus glucose10 nmol/l UAG + 8.3 mM glucoseNot change