Easy and Rapid Purification of Highly Active Nisin

Figure 2

Tricine-SDS-PAGE analysis of the cIEX purification of nisin. Purification of commercial nisin (a) and nisin secreted by the L. lactis NZ9700 strain (b). M, marker proteins; I, elution with 200 mM NaCl; II, elution with 400 mM NaCl; III, elution with 600 mM NaCl; IV, elution with 800 mM NaCl; V, elution with 1 M NaCl. Protein was visualized by silver staining. The three lowest marker proteins are indicated with molecular weights (kDa).