International Journal of Peptides / 2011 / Article / Fig 5

Research Article

Ghrelin is an Osteoblast Mitogen and Increases Osteoclastic Bone Resorption In Vitro

Figure 5

Ghrelin is weakly mitogenic to primary rat osteoblasts. Osteoblasts cultured from rat calvariae were growth-arrested in 0.1% BSA for 24 hours. Fresh media and experimental compounds were then added for a further 24 hours. (a) Thymidine incorporation: cells were pulsed with 3H-thymidine for the last 6 hours of incubation (ANOVA 𝑃 = 0 . 0 1 8 ). (b) Cell counts (ANOVA 𝑃 = 0 . 0 7 6 ). Data are mean ± SEM; *indicates 𝑃 < 0 . 0 5 compared to untreated control by t-test.
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605193.fig.005b
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