Ghrelin is an Osteoblast Mitogen and Increases Osteoclastic Bone Resorption In Vitro

Figure 5

Ghrelin is weakly mitogenic to primary rat osteoblasts. Osteoblasts cultured from rat calvariae were growth-arrested in 0.1% BSA for 24 hours. Fresh media and experimental compounds were then added for a further 24 hours. (a) Thymidine incorporation: cells were pulsed with ³H-thymidine for the last 6 hours of incubation (ANOVA 𝑃=0.018). (b) Cell counts (ANOVA 𝑃=0.076). Data are mean ± SEM; *indicates 𝑃<0.05 compared to untreated control by t-test.

(a)

(b)