International Journal of Peptides / 2011 / Article / Tab 1

Research Article

Ghrelin is an Osteoblast Mitogen and Increases Osteoclastic Bone Resorption In Vitro

Table 1

Ghrelin receptor expression in bone.

This studyMethodResult

Primary tissue
 Human osteoblasts ( 𝑛 = 1 0 ) Quantitative PCR3/10 positive
 Human bone marrow ( 𝑛 = 1 6 ) 4/16 positive
 Mouse bone marrowNegative
 Rat osteoblastic cellsNegative
 Mouse hypothalamusPositive
Cell lines
 Mouse osteoblastic MC3T3-E1Negative
 Mouse macrophage RAW264.7Negative

Kim et al. [23]

Primary Tissue
 Rat osteoblastic cellsWestern blotPositive
 Mouse osteoblastic cells
 Rat brain
Cell lines
 Mouse osteoblastic MC3T3-E1Positive
 Mouse preadipocytic 3T3-L1RT-PCR, Western blot
 Rat ROS17/2.8 osteogenic sarcoma
 Rat UMR-106 osteogenic sarcoma

Maccarinelli et al. [24]

Primary Tissue
 Rat osteoblastic cellsRT-PCR, Western blotPositive

Ueberberg et al. [25]

Primary Tissue
 Human bone marrowQuantitative PCR, IHCPositive

Delhanty et al. [27]

Primary Tissue
 Human bone marrow ( 𝑛 = 2 ) Quantitative PCRPositive

Fukushima et al. [28]

Primary Tissue
 Rat osteoblastic cellsRT-PCR, IHCPositive
 Rat femurIHCPositive
Cell lines
 Rat UMR-106 osteogenic sarcomaRT-PCRPositive

Guan et al. [35]

Primary Tissue
 Rat bone marrowRPANegative

RT-PCR: reverse transcription PCR. IHC: immunohistochemistry. RPA, ribonuclease protection assay.