Figure 3: Representative 2-DE gel image of gastric mucosa proteins from fed ad libitum (a) and 48 h fasted (b) rats. Gastric proteins were extracted from mucosa and separated on an immobilized pH 4–7 nonlinear gradient strip followed by separation on a 12% polyacrylamide gel. Silver stained gel and spots differentially expressed proteins in fed controls relative to fasted rats were picked, and analysis by mass spectrometry allowed the detection and identification of different GRp58 phosphorylated forms. Values are m e a n ± S E M of 8 rats per group.