Peptide-Modulated Activity Enhancement of Acidic Protease Cathepsin E at Neutral pH
Figure 1
Systematic of the in vitro evolution strategy for obtaining enzyme activators. (a) Progressive library. Functional peptides were identified from the first random peptide library. The second library was generated by combining peptide blocks selected from the primary library and subjecting them to the next round of selection. The third library was constructed by pairing two peptides selected from the second library. (b) Schematic representation of cDNA display-based selection-by-function. SbF-worked r/d-IVV was an RNA/DNA-type in vitro virus construct augmented with the selection-by-function construct. SbF-worked dIVV could be cleaved by cathepsin E if its binding activated cathepsin E, as shown in the box.
