Research Article
Intracellular Loop 2 Peptides of the Human 5HT1a Receptor are Differential Activators of Gi
Table 2
ic2 Peptide mimic effect on [3H]8-OH-DPAT binding. All binding inhibition values are percent of control agonist (ag.) bound. The upper portion of the table is for peptides nearer the C-terminus, including P11 from Thiagaraj et al., 2007 [53]. These peptides decreased the specific high affinity binding of 5HT1aR agonist [3H]-8-OH-DPAT by 50% at the given concentration. The lower portion of the table (P24 on) is the ic2 peptides toward the C terminus. These peptides were less effective at decreasing specific high affinity binding of [3H]-8-OH-DPAT, and values given are percent of control at the given concentration. Values for intracellular cAMP are relative to FSK-stimulated control. All values for incorporation of -[35S]-GTP into Gi are percent of control. Nominal values for control binding were 400 fmoles/mg protein.
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