Molecular Cloning and Sequence Analysis of the cDNAs Encoding Toxin-Like Peptides from the Venom Glands of Tarantula Grammostola rosea
Figure 2
Homology analysis of GTx3 series toxins. (a) Sequence alignment of GTx3 series and their related peptides. The putative signal sequence deduced by SignalP 3.0 server (http://www.cbs.dtu.dk/services/SignalP/) is indicated by dotted box. Signal sequence of MIT1 is not determined. Conserved cysteine residues are indicated by closed boxes filled with gray color. AVIT N-terminals of Prokineticin1, Prokineticin2, MIT1, and Bv8 are indicated closed boxes. (b) Phylogenetic tree of GTx3 series and related peptides. Amino acid alignment and phylogenetic tree construction were performed using the MegAlign program by Clustal W and neighbor-joining method (DNASTAR) based on the alignment (a) of mature peptides. A scale below the tree indicates the number of amino acid substitutions per 100 residues for protein sequences. Accession numbers: GTx3 series, AB201025-AB201034 and AB671309-AB671311; Bv8, AF168790; Prokineticin 1, AF333024; Prokineticin 2, AAH96695; MIT1, P25687; HWTX-XIVa1, ABY77690; ACTX-Hvf17, P81803; Pacifastacus leniusculus (Pl) astakine1, AY787656; Pl astakine2a, EF568370; Acanthoscurria gomesiana (Ag) astakine1, DR447331; Ag astakine2, DR445103; Rhipicephalus appendiculatus (Ra) astakine2, CD794853; Ixodes scapularis (Is) astakine2, EW845057; Amblyomma variegatum (Av) astakine2, BM292046; Dysdera erythrina (De) astakine2, CV178181.
