Molecular Cloning and Sequence Analysis of the cDNAs Encoding Toxin-Like Peptides from the Venom Glands of Tarantula Grammostola rosea

Table 1

Primers for PCR cloning.

Primers	Nucleotide sequence	Referred sequences
PC1	5′-TAARCGACAATGAAGAC-3′	GTx1-11, 12, 14, 15, 16
PC2	5′-TTCGATAACATGAAGAC-3′	GTx1-1, 2; GTx7-1
PC3	5′-AAAGCATGAAAACCTC-3′	GTx1-3
PC4	5′-ACTCTAAAAATGAAGGC-3′	GTx1-4, 5, 7, 8, 9
PC5	5′-TCAGCAGAAATGAAGGC-3′	GTx2-2, 3
PC6	5′-TCCATCATGAAGITNGC-3′	GTx3-4, 5, 6, 7, 8
PC7	5′-ATAACGATGAAGITINT-3′	GTx5-1
PC8	5′-GCAGCCATGAAAICINT-3′	GTx6-1
PC9	5′-GTTAAGATGAAITWYNC-3′	GTx3-1, 2, 3
PC10	5′-GCAACGATGAGRTCINT-3′	GTx4-1, 2
PC11	5′-GGAAACATGAGRAAINC-3′	GTx1-13

Oligonucleotide primers are synthesized based on the conserved signal sequences and the sequcences of 5–9 nucleotides upstream of initiation codon of the GTxs indicated in the right column. Underline indicates initiation codon, ATG. R: A/G; W: T/A; Y: T/C; N: A/T/C/G; I: inosine.