A Novel Cellular Model to Study Angiotensin II AT2 Receptor Function in Breast Cancer Cells
Validation of lentiviral vector transduction and expression in D3H2LN cell lines. (a) Flow cytometer analysis of GFP-positive cells. Grey-filled area represents noninfected parental D3H2LN cells (Ctrl), and white area represents infected D3H2LN-AT2 (AT2) cells. (b) Biochemical validation of Flag-AT2 expression by Western blotting (anti-Flag-HRP) in total cell lysate (left panel) or in anti-Flag immunoprecipitate fraction (right panel).
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