Phage Display Screening for Tumor Necrosis Factor-α-Binding Peptides: Detection of Inflammation in a Mouse Model of Hepatitis

Figure 6

TNF-𝛼 detection in liver sections of ConA-treated mice. TNF-𝛼 was first detected with a polyclonal anti-TNF-𝛼 antibody in liver sections of ConA-treated mice by a brown coloration mainly around sinusoids and blood vessels (a) as compared to untreated-liver sections (b). TNF-𝛼 was then detected with the biotinylated 2C peptide (c) by a similar brown coloration located around sinusoids and blood vessels on liver sections of treated mice. The sections of healthy liver did not show such a staining (d). The 2C peptide-USPIO-PEG also allowed for the detection of the TNF-𝛼 cytokine on liver sections of treated mice (e), while it was not detectable on healthy liver sections (f). Scale bar: 100 μm.

(a)

(b)

(c)

(d)

(e)

(f)