Phage Display Screening for Tumor Necrosis Factor-α-Binding Peptides: Detection of Inflammation in a Mouse Model of Hepatitis

Table 1

Biochemical properties of the selected peptide clones (from http://www.expasy.ch/tools/protparam.html).

Clone	Sequence	T1/2 (hours)	pI
C2-R3	C-GSKTQAP-C	30	8.06
C18-R3	C-GLPWLST-C	30	5.51
C22-R3	C-STPHNLG-C	1.9	6.72
C25-R3	C-LATGNQI-C	5.5	5.51
C30-R3	C-PATLTSL-C	20	5.51
C33-R3	C-HGAPNRL-C	3.5	8.08
C41-R3	C-RPPIGAF-C	1	8.07
C42-R3	C-TSQSQHM-C	7.2	6.72
C45-R3	C-GPAVYMK-C	30	8.05
C46-R3	C-QGDLPGY-C	0.8	3.80
C32-R4	C-SPHTTIA-C	1.9	6.72
C33-R4	C-EPFAGRS-C	1	5.99
C35-R4	C-TSPLPGT-C	7.2	5.51
C46-R4	C-SYEAHQT-C	1.9	5.24
C47-R4	C-NNPLKSL-C	1.4	8.06

T_1/2: half-life theoretically estimated in mammalian reticulocytes in vitro and based on the N-end rule [20].
pI: isoelectric point which is the pH at which a particular molecule or surface carries no net electrical charge.