Total Chemical Synthesis of a Heterodimeric Interchain Bis-Lactam-Linked Peptide: Application to an Analogue of Human Insulin-Like Peptide 3
Figure 5
RP-HPLC and ESI-MS data of ΔA25/26 human INSL3 bis-lactam A11-B10/A24-B22. (a) RP-HPLC of crude Fmoc-AspA10 ΔA25/26 INSL3 A11-B10/A24-B22; (b) RP-HPLC of purified ΔA25/26 human INSL3 bis-lactam A11-B10/A24-B22; (c) ESI-MS of purified ΔA25/26 human INSL3 bis-lactam A11-B10/A24-B22. HPLC detection wavelength = 220 nm.