Nociceptin Signaling Involves a Calcium-Based Depolarization in Tetrahymena thermophila
Figure 2
Calcium chelators inhibit the behavioral response to 50 μM nociceptin-NH2 in Tetrahymena thermophila. EGTA (closed circles) reduces avoidance to 20% (near baseline) at a concentration of 50 μM. The IC50 of EGTA is approximately 7.5 μM. Thapsigargin (open triangles) reduced avoidance by 50% at a concentration of 100 μM; however, increasing the concentration to 300 μM did not cause a significant decrease in avoidance beyond that seen with 100 μM thapsigargin. . represents the number of trials conducted. Each trial consisted of 10 cells, which were individually scored as positive or negative for avoidance.
