Netrin-1 Peptide Is a Chemorepellent in Tetrahymena thermophila

Table 3

A comparison of polycationic peptides and proteins which are chemorepellents in Tetrahymena. Calcium is required for avoidance of all of the compounds listed except for netrin-1 peptide, suggesting a unique signaling mechanism for this peptide. Netrin-1 peptide also has one of the lowest EC₁₀₀ values of the polycationic compounds studied to date, suggesting a high affinity for its putative receptor.

Chemorepellent	Charge at pH 7.0	EC100	Calcium required for avoidance?	Reference
Lysozyme	+11	100 μM	Yes	Kuruvilla et al., 1997 [9]; Kuruvilla and Hennessey, 1998 [14]
PACAP-38	+11	0.1 μM	Yes	Mace et al., 2000 [12]
Nociceptin	+4	100 μM	Yes	Lampert et al., 2013 [13]
Netrin-1 peptide	+6	1 μM	No	Current study

Electrophysiological studies done. Results obtained using pharmacological inhibitors only.