ELISA indicates that a netrin-like protein is secreted by Tetrahymena thermophila. A polyclonal anti-netrin-1 antibody showed reactivity in ELISA with whole cell extract as well as secreted protein obtained from Tetrahymena thermophila. Using a standard curve of netrin-1 concentration, we found that both total protein and secreted protein had a netrin-like peptide concentration of approximately 0.1 μM.
|Protein extract||Total protein concentration||Concentration of netrin-like peptide|
|Whole cell extract||114 mg/mL||0.1 μM|
|Secreted protein||0.29 mg/mL||0.1 μM|