International Journal of Peptides / 2016 / Article / Tab 4

Research Article

Netrin-1 Peptide Is a Chemorepellent in Tetrahymena thermophila

Table 4

ELISA indicates that a netrin-like protein is secreted by Tetrahymena thermophila. A polyclonal anti-netrin-1 antibody showed reactivity in ELISA with whole cell extract as well as secreted protein obtained from Tetrahymena thermophila. Using a standard curve of netrin-1 concentration, we found that both total protein and secreted protein had a netrin-like peptide concentration of approximately 0.1 μM.

Protein extractTotal protein concentrationConcentration of netrin-like peptide

Whole cell extract114 mg/mL0.1 μM
Secreted protein0.29 mg/mL0.1 μM

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