GFP-Atg8/LC3 dot accumulation and TEM method to detect autophagic activity. (a) LC3 dot formation upon starvation in fibroblasts isolated from GFP-Atg8/LC3 transgenic mice. The green dots are autophagic vesicles labelled by GFP-Atg8/LC3. (b) Transmission electron microscopic picture of an autophagic vesicle (arrow) in kidney of tunicamycin injected mouse. Note that in addition to cytoplasmic material, a mitochondrium (arrowhead) is also engulfed inside the double membrane vesicle.