Molecular Cytogenetic Mapping of Chromosomal Fragments and Immunostaining of Kinetochore Proteins in Beta

Figure 2

Blue fluorescence in each panel shows the chromosomes stained with DAPI. The scale bar for left and central panels in (Q) represents 10 µm. Green fluorescence shows hybridization of the ribosomal gene probe pTa71. The chromatids of the chromosome fragments are schematically contoured in right panels. FISH with the telomeric probe (TTTAGGG)n (red) and on (A) B. procumbens; (B) PRO1;. (C) PRO1 chromosome fragment; (D) B. patellaris; (E) PAT2; (F) PAT2 chromosome fragment. The subtelomeric satellite repeat pRp34 (red) cloned from B. procumbens is found on (G) B. procumbens; (H) PRO1 and its chromosome fragment (I); (J) B. patellaris; (K) PAT2; (L) close-up of the panel (H). The dispersed repeat pAp4 specific to the Procumbentes localized by FISH on (M) B. procumbens; (N) on the PRO1 chromosome fragment; (O) close-up of the panel (N); (P) B. patellaris; (Q) on PAT2 is limited to the chromosome fragment, where it shows a weak dispersed distribution (R).

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