Agrobacterium-Mediated Gene Transfer to Cereal Crop Plants: Current Protocols for Barley, Wheat, Triticale, and Maize
Reporter gene expression in immature embryo-derived calluses for three weeks (barley), four weeks (wheat), two weeks (triticale), 12 d (maize, upper picture) and 8 weeks (maize, lower picture) after co-culture. On the lower pictures of barley, wheat and triticale, the same objects are shown as above, but being exposed to far blue light and recorded with a GFP-filter set. The calluses of maize are shown following histochemical GUS assay .
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