Identification of Aluminum Responsive Genes in Al-Tolerant Soybean Line PI 416937

Table 2

Aluminum regulated genes in soybean genotype PI 416937 48 h post treatment^†.

Unigene ID	Fold change	Average±SD	Functional category	Annotation	𝑒-value
Gma.6089	226.57 (up)	6.48±1.80	Unclassified	Unknown	—
BM139770	176.22 (up)	5.91±0.80	Unclassified	Unknown	—
Gma.2586	154.32 (up)	5.86±0.94	Unclassified	Unknown	—
Gma.1654	130.42 (up)	4.69±0.64	Transport	Coatomer protein complex subunit 2 protein transporter	2𝑒−51
Gma.26937	115.43 (up)	5.43±1.37	Unclassified	Unknown	—
Gma.35222	113.94 (up)	4.67±0.56	Stress response	Syringolide-induced protein B13-1-9 defense protein	4𝑒−64
Gma.24062	89.44 (up)	5.15±1.37	Unclassified	Unknown	—
Gma.8048	79.07 (up)	5.03±0.94	Unclassified	Unknown	—
Gma.27466	72.87 (up)	4.96±1.57	Unclassified	Unknown	—
Gma.12326	71.14 (up)	4.84±1.90	Unclassified	Unknown	—
Gma.6948	69.75 (up)	4.69±1.27	Unclassified	Unknown	—
BU551397	65.63 (up)	4.37±0.70	Unclassified	Hypothetical protein	2𝑒−15
Gma.2523	61.59 (up)	4.78±0.96	Stress response	Secretory protein (R14 protein soybean-defense protein)	6𝑒−64
Gma.35601	59.51 (up)	3.61±0.43	Transport	Heavy-metal transport/detoxification	2𝑒−19
Gma.6948	58.09 (up)	4.45±0.98	Unclassified	Unknown	—
Gma.16246	55.90 (up)	4.27±0.59	Unclassified	BAP2 (BON associated protein 2)	1𝑒−15
Gma.30731	54.43 (up)	4.14±0.90	Unclassified	Unknown	—
BI967874	53.43 (up)	4.4±1.35	Unclassified	Unknown	—
Gma.4526	50.59 (up)	4.38±1.02	Transcription factor	Zinc finger (C2H2 family protein)	7𝑒−27
Gma.25191	47.41 (up)	5.06±1.11	Unclassified	Unknown	—
Gma.9397	47.24 (up)	4.28±0.98	Stress response	Syringolide-induced protein B13-1-9/defense protein	1𝑒−53
Gma.25462	46.34 (up)	4.34±0.65	Transcription factor	WRKY19 DNA-binding protein 19	1𝑒−62
BU579058	45.35 (up)	4.44±1.44	Metabolism	N-acetyltransferase activity	2𝑒−40
Gma.28852	43.12 (up)	4.10±0.58	Metabolism	Cytochrome P450	0.0
Gma.27514	41.91 (up)	3.91±0.98	Stress response	Basic secretory protein/defense protein	3𝑒−69
Gma.23347	41.67 (up)	4.08±1.79	Unclassified	Unknown	—
Gma.22079	41.46 (up)	4.34±0.70	Stress response	Glutathione s-transferase	5𝑒−57
Gma.32994	40.77 (up)	3.71±0.62	Stress response	Acidic endochitinase (chitinase III-A)	7𝑒−93
Gma.27743	39.93 (up)	4.14±0.98	Unclassified	Unknown	—
Gma.1622	39.56 (up)	3.93±1.02	Unclassified	Hypothetical protein/ABC transporter like	8𝑒−31
Gma.36756	36.68 (up)	4.27±1.08	Transcription factor	WRKY17 protein/transcription factor	1𝑒−101
Gma.5622	32.77 (up)	4.29±0.75	Unclassified	Unknown	—
Gma.26204	32.06 (up)	3.82±0.46	Metabolism	Transferase/transferase activity	2𝑒−29
Gma.27239	31.70 (up)	3.83±1.35	Unclassified	Unknown	—
Gma.36287	31.08 (up)	4.56±0.48	Metabolism	Carboxylesterase/lipase activity	3𝑒−64
Gma.28246	30.45 (up)	3.54±0.64	Unclassified	Unknown	—
Gma.36753	30.41 (up)	4.50±0.74	Transcription factor	WRKY 30(DNA binding protein	1𝑒−104
Gma.8565	29.01 (up)	3.45±0.70	Metabolism	Hydrolase /xyloglucan endotransglycosylase	5𝑒−18
Gma.7861	28.48 (up)	3.48±1.34	Unclassified	Unknown	5𝑒−20
Gma.32790	28.00 (up)	3.63±0.99	Stress response	Band 7 family protein/hypersensitive inducible reaction protein 1	3𝑒−27
Gma.7697	27.48 (down)	−3.66±0.81	Unclassified	Hypothetical protein/31 kDa glycoprotein	2𝑒−137
Gma.31827	27.44 (up)	4.22±0.65	Transcription factor	WRKY70/DNA-binding protein 70	5𝑒−87
Gma.21022	27.36 (up)	4.13±1.18	Unclassified	Unknown	—
Gma.28273	27.29 (up)	3.84±0.87	Transcription factor	NAC6 /NAC domain protein/apical elongation plant development protein	2𝑒−156
Gma.35830	27.20 (up)	3.48±0.77	Signaling	Regulation of gene silencing/calcium-sensor	9𝑒<?𝑏ℎ𝑙𝑡?>−<?ehlt?>33
Gma.8262	26.92 (up)	3.73±1.33	Stress response	AGc 2-1(oxidative signal-inducible kinase	3𝑒−57
Gma.14080	26.50 (up)	3.81±0.80	Transport	Similar to ATMRP3/multidrug resistance glutathione s-conjugate-exporting ATPase	8𝑒−11
Gma.27371	25.99 (up)	3.78±1.33	Unclassified	Unknown	—
BM523736	25.34 (up)	3.76±0.83	Metabolism	Transferase family protein	1𝑒−23
Gma.28330	25.32 (up)	3.84±1.53	Unclassified	Calcium-binding protein	7𝑒−23
Gma.34717	24.87 (up)	3.83±1.15	Unclassified	Unknown	—
Gma.26682	24.58 (up)	3.95±1.15	Unclassified	Unknown	—
Gma.17184	24.42 (up)	3.77±0.52	Transport	Glycolipid-binding/transport protein	2𝑒−57
Gma.11888	24.39 (up)	3.19±0.25	Transport	ATPP2-B10 (pheloem protien2) carbohydrate binding	6𝑒−27
Gma.4222	23.57 (up)	3.14±0.97	Unclassified	Hypothetical protein	2𝑒−57
Gma.26712	23.39 (up)	2.82±0.18	Unclassified	Unknown	—
Gma.33327	23.16 (up)	3.56±0.29	Transcription factor	Transcription factor	2𝑒−166
Gma.17019	23.08 (up)	3.67±1.54	Unclassified	Unknown	—
Gma.33178	23.07 (up)	3.81±1.54	Unclassified	Plastocyanin-like domain-containing copper ion binding	3𝑒−33
Gma 35364	22.81 (up)	3.55±0.40	Stress response	FAD-linked oxidoreductase 1/carbohydrate-oxidase	1𝑒−52
Gma.15839	22.63 (down)	−4.00±0.36	Metabolism	GDSL-motif lipase/hydrolase	2𝑒−39
Gma.6948	22.54 (up)	3.73±1.24	Unclassified	Unknown	—
Gma.2821	22.36 (up)	3.40±0.95	Stress response	PR-5 protein (pathogenesis related)	4𝑒−134
Gma.8628	21.20 (up)	3.19±1.11	Unclassified	Unknown protein	—
BQ473604	20.51 (up)	3.43±0.83	Unclassified	Hypothetical protein	8𝑒−46
Gma.9913	20.34 (up)	3.81±0.44	Unclassified	Unknown protein	—
Gma.144	20.01 (up)	3.72±1.00	Transport	Nodulin protein/transport function	0.0
Gma.34099	19.67 (up)	2.93±0.93	Unclassified	Hypothetical protein	2𝑒−77
Gma.4305	19.63 (up)	3.82±0.62	Stress response	Glutathione s-transferase (GST 15)	7𝑒−128
Gma.4336	19.56 (up)	3.16±0.98	Unclassified	Unknown	—
Gma.24625	19.40 (up)	3.50±1.12	Transport	Heavy metal transport/detoxification	2𝑒−20
Gma.7726	19.18 (up)	3.36±1.22	Signalling	Calcium-binding EF hand family protein	4𝑒−25
Gma.26531	18.95 (up)	3.55±1.31	Transcription factor	Zinc finger (C3HC4-type ring familyn)	2𝑒−26
Gma.34717	18.94 (up)	3.46±1.24	Unclassified	Unknown	—
BE822282	18.64 (up)	2.87±0.66	Unclassified	Unknown	—
Gma.27062	18.22 (up)	2.96±0.95	Transcription factor	NAc domain containing protein 2 plant development/apical elongation	3𝑒−103
Gma.4478	18.03 (up)	3.54±1.03	Unclassified	Hypothetical protein	2𝑒−10
Gma.24807	18.01 (up)	3.09±0.76	Unclassified	Unknown	—
BK000119.1	17.98 (up)	3.36±0.87	Cell cycle	Phytosulfokines 4 precursor/ growth factor cell differentiation, cell proliferation	3𝑒−19
Gma.29479	17.92 (up)	3.16±0.52	Unclassified	Unknown	—
Gma.10956	17.76 (up)	3.32±0.74	Stress response	Similar to pathogenesis-related protein (STH-2)	1𝑒−40
BI967589	17.25 (up)	3.08±0.49	Unclassified	Unknown	—
Gma.17184	17.16 (up)	2.94±0.11	Transport	Heavy-metal-associated domain containing protein metal ion transport	1𝑒−10
Gma.24561	17.08 (up)	3.15±0.31	Unclassified	Unknown	—
Gma.21739	17.05 (up)	3.33±0.82	Metabolism	AAA-type ATpase protein/ATpase activity	5𝑒−39
Gma.17184	16.89 (up)	3.27±0.63	Transport	Glycolipid-binding protein/glycolipid transport
Gma.4366	16.62 (up)	3.29±1.27	Metabolism	VTc2 (Vitamin C defective 2)/ L-ascorobic-acid biosynthesis	1𝑒−36
Gma.26405	15.37 (up)	3.04±0.83	Unclassified	Unknown	—
DQ222982	15.09 (up)	3.42±1.04	Transport	Lipocalin/ fatty acid transport	2𝑒−107
Gma.17929	15.05 (up)	3.12±0.58	Metabolism	Transferase family protein	8𝑒−76
Gma.21512	14.99 (up)	2.71±0.78	Unclassified	Unknown	—
BE440732	14.85 (up)	3.48±0.97	Unclassified	Unknown	—
Gma.29663	14.64 (up)	2.52±1.04	Unclassified	Unknown	—
Gma.31861	14.60 (up)	3.09±1.07	Unclassified	Unknown	—
Gma.29655	14.52 (up)	3.00±0.34	Metabolism	CytochromeP50 subfamily B polypeptide 1	3𝑒−58
Gma.11257	14.51 (up)	2.65±0.86	Unclassified	Hypothetical protein exo-1, 3-beta-glucanase precursor	9𝑒−64
Gma.26640	14.48 (up)	2.93±1.18	Unclassified	Unknown	—
Gma.28243	14.18 (up)	3.28±0.85	Unclassified	Unknown	—
CD394418	14.12 (up)	3.08±1.24	Metabolism	Ribulose-1, 5-bisphosphate carboxylase	2𝑒−30
Gma.1527	14.09 (down)	−2.26±1.40	Metabolism	Dihydroflavonol reductase (anthocyanin biosynthesis)	0.0
Gma.25234	14.08 (up)	2.96±0.18	Transcription factor	WRKY43 protein	4𝑒−131
Gma.28057	13.62 (up)	2.52±0.68	Transcription factor	Sali5-4a protein (ADR6)	8𝑒−60
Gma.8480	13.58 (up)	3.20±0.69	Stress response	Resistance protein LM12	0.0
Gma.28756	13.53 (up)	2.89±0.53	Unclassified	Unknown	—
BM177218	13.45 (up)	3.14±0.45	Unclassified	Unknown	—
BG551078	13.37 (up)	3.09±0.42	Unclassified	Conserved hypothetical protein	6𝑒−11
Gma.728	13.28 (up)	2.78±0.99	Unclassified	Unknown	—
Gma.35332	13.23 (up)	3.45±0.73	Unclassified	Unknown	—
Gma.26712	13.17 (up)	3.09±1.01	Unclassified	Unknown	—

^† Significance threshold (𝑃<.01, Fold change >= 3); up: upregulated, down: downregulated; 𝑒-value: the probability that the match between the gene and its annotation has no biological basis. Fold change: absolute value of the ratio of gene expression under Al to gene expression of untreated control. SD: standard deviation.