Proteomic Analysis of Soybean Roots under Aluminum Stress

Table 2

Aluminum regulated-proteins detected in soybean cultivar Young in time-course Al stress Experiment.

Spot	Accession	Protein ID/functional class^a	Average expression fold change^b
			6 h	SE	51 h	SE	72 h	SE
3	gi/27883937	Phosphoribosylformayl glycinamidine synthase (PM)	−1.34	0.13	1.33	0.10	1.45	0.20
52	gi/224160640	Zinc Knuckle (UN)	1.24	0.18	nd	0.17	1.67	0.17
87	gi/11385431	Glutathione (DD)	nd	—	1.22	0.08	4.34	1.04
100	gi/156938901	Profilin/actin binding (CS)	1.92	0.8	nd	—	nd	—
103	gi/79321519	Heat shock protein 70 (PD)	−1.02	1.10	1.08	1.10	1.44	0.06
104	gi/153805634	Unknown (UN)	nd	—	nd	—	1.38	0
106	gi/15219879	Apoptotic ATpase (DD)	−1.08	0.01	1.15	0.11	1.44	0.10
107	gi/30696901	Xylose isomerase (PM)	1.05	0.01	nd	—	1.37	0.09
109	gi/168030956	Serine/threonine∖protein kinase (ST)	nd	—	nd	—	1.31	0.04
112	gi/436169	Lipoxygenase (PM)	−1.10	0.03	1.13	0.05	1.35	0.23
113	gi/77552532	Unknown (UN)	−1.52	0.62	1.21	0.06	1.38	0.18
116	gi/2232254	NADH:flavin oxidoreductase (UN)	−1.36	0.06	nd		1.49	0.18
118	gi/223541365	Transitional endoplasmic reticulum ATPase (CS)	nd	—	1.37	0.03	1.37	0.27
119	gi/223974443	Unknown (UN)	−1.05	0.01	−1.03	1.07	1.31	0
133	gi/42820320	Copper amino oxidase (DD)	1.03	1.05	nd	—	1.35	0.07
136	gi/195636596	T-complex protein 1 alpha (PD)	1.06	0.02	1.35	0	1.05	1.37
137	gi/125574688	Glutathione-s-transferase (DD)	1.19	0.17	1.21	0.08	1.33	0.35
140	gi/74053562	Tubulin beta-3-chain (CS)	−1.03	0.01	1.02	0.08	1.62	0.3
147	gi/5225811	Unknown (UN)	1.03	0.03	1.05	1.06	−1.39	0.01
151	gi/124112056	Septum site determining protein (CS)	nd	—	1.52	0.53	−1.36	0.05
155	gi/87240711	V-ATPase subunit C (DD)	nd	—	nd	—	1.31	0.23
156	gi/1346698	Phosphoglycerate kinase (PM)	−1.03	0	1.14	1.20	1.45	0.54
157	gi/1582580	Caffeic acid O-methyl transferase (SM)	−1.04	1.06	nd	—	1.35	0.09
158	gi/1346028	Polyprenyl synthetase (SM)	1.09	1.09	1.06	0.04	1.32	0.05
166	gi/3023197	14-3-like (ST)	nd	—	1.09	0.04	−1.32	0.23
167	gi/393401	Alpha tubulin (CS)	1.21	0	1.41	0.06	−1.42	0.20
172	gi/78146198	MADS box protein/transcription factor (TF)	1.11	0.11	nd	—	1.85	0.74
185	gi/115511406	Acirduyctase dioxygenase (PM)	1.25	0.13	nd	—	1.61	0.01
186	gi/224105487	Unknown (UN)	1.11	1.22	1.22	0.21	1.34	0
187	gi/145352433	Unknown (UN)	−1.01	1.02	1.44	0.01	1.59	0.25
188	gi/17380185	Proteasome subunit beta type-1 (PD)	1.04	0.02	nd	—	1.72	0.11
191	gi/223543735	Proteasome subunit beta type (PD)	−1.04	0.02	nd	—	1.74	0.52
193	gi/145340582	Unknown (UN)	1.08	0	nd	—	1.41	0.08
195	gi/209778987	Putative rab1C protein (IT)	−1.05	1.15	−1.02	1.10	−1.67	0.29
196	gi/18395025	20S proteasome beta subunit C (PD)	1.07	1.16	nd	—	1.32	0.27
197	gi/125577605	DNJ/HSP40 (DD)	1.04	1.16	1.00	1.07	1.35	0.31
198	gi/34485411	Resistance protein (DD)	1.00	1	1.40	0.06	1.73	0.37
200	gi/20140683	Translationally-controlled tumor protein homolog (CD)	nd	—	−1.53	0.08	−1.46	0.10
201	gi/40644130	Allene oxide cyclase (PM)	−1.51	0.47	nd	—	1.59	0.26
204	gi/223510245	Unknown (UN)	1.04	0.04	1.01	1.17	1.32	0.05
206	gi/197294157	Hypothetical protein (UN)	−1.53	0.55	nd	—	1.42	0.22
212	gi/110931690	MYB transcription factor (TF)	1.39	0.38	−1.93	0.75	nd	—
214	gi/18143656	Mcp20 (PD)	1.35	0.18	nd	—	1.61	0.73
218	gi/829282	Eukaryotic initiation factor A (PS)	1.93	0.46	nd	—	nd	—
220	gi/15238219	Unknown (UN)	nd	—	−1.32	0.08	−1.72	0.08
225	gi/223547693	Electron transporter (E)	nd	—	−1.02	1.07	2.10	0.20
226	gi/145345251	Glycogen synthase kinase-3 (PM)	nd	—	nd	—	1.77	0.21

^aPM: primary metabolism, SM: secondary metabolism, E: energy, PD: protein destination, TF: transcription factor, CS: cell structure, ST: signal transduction, DD: disease/defense, t, IT: intracellular trafficking, CD: cell division, UN: unknown function;
^bexpression fold change at each time point, negative values: downregulation, positive values: upregulation, fold change significance cut-off is ±1.3, SE: standard error, nd: not detected.