Figure 5: (a) Deduced amino acid sequences of wheat, rice, and Arabidopsis F3H. Lines with an arrowhead indicate the intron insertion sites. The region in the black box indicates a characteristic motif, pfam03171, of 2OG-Fe(II) oxygenase superfamily. White circles indicate conserved histidine (H) and aspartic acid (D) residues for ferrous-iron coordination, and a black circle indicates arginine (R) residue for a binding site of 2-oxoglutarate. Triangles show a site of glutamine (Q) residue lacked and an amino acid substitution from threonine (T) to alanine (A) in F3H-A1. (b) UPGMA tree depicted using GENETYX software ver. 7.0, using the following genes belonging to 2OG-Fe(II) oxygenase superfamily: ANSs of Arabidopsis thaliana (Q96323) and Oryza sativa (CAA69252), FLSs of Arabidopsis thaliana (Q96330) and Oryza sativa (XP_467968), F3Hs of Arabidopsis thaliana (Q9S818), Hordeum vulgare (CAA41146), Ipomea nil (BAA21897), Medicago sativa (S71772), Oryza sativa F3H (XP_474226), Vitis vinifera (P41090), and Zea mays (AAA91227), and F3H-A1 (AB223024), F3H-B1 (AB223025), and F3H-D1 (AB223026) of Triticum aestivum.