Differential Expression of Three Flavanone 3-Hydroxylase Genes in Grains and Coleoptiles of Wheat

Table 2

Names and sequences of the primers used in the experiments.

Primer	Sequence (5′-3′)	Sequence source	Accession No.
Primers for genomic DNA
F3H3LP	GCGACACAAGTGGACGAT	whe24e20	BJ237068
F3H2RP	GAACGTCGCGATCGACAG	barley F3H (Meldgaard, 1992)	X58138
Primers for 3′ region
F3HLP	CCTACTTCTCGTACCCGGTG	barley F3H (Meldgaard, 1992)	X58138
F3H2LP	ATTCGTCGTCAACCTCGG	barley F3H (Meldgaard, 1992)	X58138
Oligo (dT) with 3′ adapter	GGCCACGCGTCGACTAGTACTTTTTTTTTTTTTTTTT
3′ adapter	GGCCACGCGTCGACTAGTAC
F3H-3UTRRP	TCTGTCAGACACATGCACACA	3′ region obtained by 3′ RACE
F3Hint1LP	ACTGTCTTGTAGCCGCTTCC	1st intron of F3H-A1, B1
Primers for RT-PCR
F3H5LP	CAAGAAGCAGGCCAAGGAC	3rd exon of F3H-A1, B1, D1
F3HARP	CCAAACTCACGATAACTCCTTATTTAC	3′ regions of F3H-A1
F3HBRP	GGAGAATAATCAATCCCACCA	3′ regions of F3H-B1
F3HDRP	CTGCTACACACGTACGGATACC	3′ regions of F3H-D1
Primers for inverse PCR and
chromosomal location analysis
F3H1stintAspLP	TGCTAGAATGGCGGTGGGT	1st intron of F3H-A1
F3H1stintBspLP	GATGATGGTGGGGAACGGT	1st intron of F3H-B1
F3Hint2LP	GCCATGCCACGTAAAATGAT	1st intron of F3H-D1
F3HABDRP	CTTCACCGGGTACGAGAAGT	2nd exon of F3H-A1, B1, D1
F3HBLP	GCAGGTATACACGCATTCATTT	1st exon and intron of F3H-B1
F3H3RP	GTGGCTGGAGACGATGAAG	whe24e20	BJ237068
F3H4LP	CGATACAGCGAGCGACTCAT	2nd exon of F3H-A1, B1, D1
F3H4RP	AGGAACGTCTCGTTGCTCAC	1st exon of F3H-A1, B1, D1
F3H5RP	TTGTGGTTTTCTGGACGTTG	5′ regions of F3H-A1, B1, D1
F3HABDLP	GACAAGCTCCGGTACGACAT	1st exon of F3H-A1, B1, D1
Primers for Actin
TaActinLP	GAGGGATACACGCTTCCTCA	wheat actin	AB181991
TaActinRP	GAAAGTGCTAAGAGAGGCCAAA	wheat actin	AB181991