Differential Expression of Three Flavanone 3-Hydroxylase Genes in Grains and Coleoptiles of Wheat

Table 3

Primers and annealing temperature for each PCR.

	F3H-A1	F3H-B1	F3H-D1
Location analysis
Sense	F3H1stintAspLP	F3H1stintBspLP	F3Hint2LP
Antisense	F3HABDRP	F3HABDRP	F3HABDRP
Temp.^*1	62.0	62.0	62.0
Expression analysis
Sense	F3H5LP	F3H5LP	F3H5LP
Antisense	F3HARP	F3HBRP	F3HDRP
Temp.^*1	55.0	60.0	62.0
Inverse PCR
Sense	F3HBLP	F3HABDLP	F3H4LP	1st PCR
Antisense	F3H3RP	F3H4RP	F3HABDRP
Temp.^*1	55.0	55.0	55.0
Sense	F3H1stintAspLP	F3HBLP	F3H2LP	2nd PCR
Antisense	F3H4RP	F3H5RP	F3H3RP
Temp.^*1	58.0	58.0	58.0
Sense	F3H1stintAspLP	F3H1stintBspLP	F2H2LP	3rd PCR
Antisense	F3H5RP	F3H5RP	F3H4RP
Temp.^*1	60.0	60.0	60.0

^*1Annealing temperature at PCR reaction.