Table 3: Primers and annealing temperature for each PCR.

  F3H-A1F3H-B1F3H-D1 

Location analysis
 SenseF3H1stintAspLPF3H1stintBspLPF3Hint2LP
 AntisenseF3HABDRPF3HABDRPF3HABDRP
 Temp.*162.062.062.0
Expression analysis
 SenseF3H5LPF3H5LPF3H5LP
 AntisenseF3HARPF3HBRPF3HDRP
 Temp.*155.060.062.0
Inverse PCR
 SenseF3HBLPF3HABDLPF3H4LP1st PCR
 AntisenseF3H3RPF3H4RPF3HABDRP
 Temp.*155.055.055.0
 SenseF3H1stintAspLPF3HBLPF3H2LP2nd PCR
 AntisenseF3H4RPF3H5RPF3H3RP
 Temp.*158.058.058.0
 SenseF3H1stintAspLPF3H1stintBspLPF2H2LP3rd PCR
 AntisenseF3H5RPF3H5RPF3H4RP
 Temp.*160.060.060.0

*1Annealing temperature at PCR reaction.