International Journal of Plant Genomics

Research Article

Advances towards a Marker-Assisted Selection Breeding Program in Prairie Cordgrass, a Biomass Crop

Table 2

Summary and description of primer sequences designed for SSR regions identified in prairie cordgrass and their amplification products.
LocusLibraryPrimer sequence (5′3′)SSR motifExpected
size
(bp)		Size
range
(bp)		  
(°C)		 GenBank ID
SPSD001 F: GCTGCTCCTCTTCTCTGTCT(CA)13180212–38655GQ354531
R: ACGGCACACTTAGTTTTCTGPerfect   
SPSD002 F: GCACTGTTTGGTGATGCTC(CA)13249100–65555GQ354532
R: CTGACGCAAGGTTGATGAGPerfect   
SPSD009 F: ATGGTTTCACAAGTCGGAAGT(GT)15166215–43255GQ354533
R: CAGGGCTGCCTACAAGATGPerfect   
SPSD010 F: AACCAAAAGGATAGACCCTA(CA)28168127–31053GQ354534
R: ACGAAATATGTGACCGATACPerfect   
SPSD011 F: CTAACGTATGTCGTTCATGTGG(GT)11135147–25153GQ354535
R: AAGGCGATTTTAAGAGGCTAAGPerfect   
SPSD013 F: GGGATGCTTTGTAGATAAGAAA(CA)4115785–17355GQ354536
R: TCTTCCTCTTTACTCTGTCACCPerfect   
SPSD016 F: CCGACTACGAGCCACATT(CA)14155156–26853GQ354537
R: GTTCCACACATACGAAGGAGAPerfect   
SPSD019 F: CCTGCTTACTCTTACTCCGTC(CA)13(GA)7134220–36553GQ354538
R: ACCCTTTTTTCTTTTGGTCTCCompound   
SPSD020 F: ATGAGACGATAGCAGGATGAC(CA)23178146–28855GQ354539
R: AGCAGATTACGATTCAGATGGPerfect   
SPSD025 F: CAGTCCATGCAACTCAGAAGTA(CT)10(CA)38269204–74855GQ354540
R: AACCTGATAGAAGTGGTCATGCCompound   
SPSD026 F: GTGGAATCAACAACACCAGA(GA)13(GT)20209196–53955GQ354541
R: GTCGCTTTAGCCCGTAAGCompound   
SPSD003 F: ATGGAAACTGTCTGGAACTGAC(CT)28294231–26355GQ354542
R: AGCAATAACCACAGAAGAGACCPerfect   
SPSD027 F: TCAAACAATGGCGGAGAAG(CT)17214188–22455GQ354543
R: CTGGCTCCACCTCTTTGGPerfect   
SPSD004 F: GTTGCTCGGTTCCAGTTG(GA)22178133 & 16455GQ354544
R: CGCCACACAAAAGTAGCCPerfect   
SPSD031 F: TCGCACTTTTGATTCTCTTTAC(CT)20188155–34653GQ354545
R: TGGATGGATTAGGTTACTGTTGPerfect   
SPSD032 F: CTCTCGCCCATTGCTACTTA(CT)13196146–19353GQ354546
R: CCATTGCTATGTTGTTTGAGCPerfect   
SPSD034 F: CAGGTCTACGGAGGTCACTAC(CT)12160144–30653GQ354564
R: TCAAAAGAAGAGCACATACACAPerfect   
SPSD036 F: TTCACCACACCACTTTATCC(CT)29271222–26053GQ354547
R: GGAAGCAACAAACATTGATGPerfect   
SPSD039 F: CTTTCAGATAGCTCCACTGATC(GA)12206193–44855GQ354548
R: AGCAATAACTGTGCATACCTCTPerfect   
SPSD040 F: AATCGAAGTAGCAGACACCAAC(GA)11214190–41655GQ354549
R: CATGCGTTTTTCACTCATGTAGPerfect   
SPSD041 F: CCCAACGATGATTTCTCTTG(GA)26135104–18053GQ354550
R: TCACGGTAACACGATTAGTCCPerfect   
SPSD042 F: ACCTCCCACTCGTTGCTAC(CT)19211131–25753GQ354551
R: GCCATTGCTCTGTTGTTTGPerfect   
SPSD043 F: GTTCAAATGCGAACAAATCAG(GA)30277231–25253GQ354552
R: ATTCGATCTCACATGCAACACPerfect  
SPSD044 F: AGCTATATGACCCGAGACTGTG(GA)20275254–27655GQ354553
R: GGAATGGTCCCATACTTAATCCPerfect   
SPSD045 F: AACGGAGGAAGTAATAAATAGC(CT)16241214–42653GQ354554
R: AGCACACACTAGCAAGGACPerfect   
SPSD046 F: CAGGTTTATCAGTGAAGACATC(TA)9(GA)1227857–42653GQ354555
R: GAGGTTCTTAAAGGAACATAGCCompound   
SPSD047 F: CCACCTTCCTTGGATACAC(CT)17194156–32653GQ354556
R: CCACAACTACCACCTCPerfect   
SPSD005 F: TGAACCAACATAACCTACCTG(TTC)19297192–45255GQ354557
R: CCACACTAAACCGAAACTTGPerfect   
SPSD048 F: AAGGGCATAGTTTCAACCAAG(AAG)101978855GQ354565
R: CTTTTGCTTGTTCATCAACATGPerfect   
SPSD007 F: AATCCTTCGCCTATCCTACAC(CTG)8(CT)9168146–51955GQ354558
R: TTCACACAGCAGACAGAACTGCompound   
SPSD008 F: GCAAGAACAGACTCAAGAGC(CAG)9(CAA)4(CAG)4(CAA)2276144–30955GQ354559
R: CTGCTGCTGAAGTAAAAGTTGCompound   
SPSD049 F: TGGATTGTTTCCTGATACTCCA(TTG)5(CTG)5299303–66753GQ354560
R: CCATAAATTGCTGCATTATTCCCompound   
SPSD050 F: GAAGCAGAAAACACAGTATTGC(CTG)5225225–50553GQ354561
R: TTGCTGGAATTTAACCTATCTGPerfect   
SPSD053 F: ACGCCTTCTTCACTCCAAC(CTG)8208166–31653GQ354562
R: GCCACCAGTTTTCATCACCPerfect   
SPSD056 F: GTTCTCCAAAGTCTCCTCCT(TCC)6TTC(TCC)219779–23053GQ354563
R: ATCTTTACCTTCCTTCTGGGInterrupted