Local Assemblies of Paired-End Reduced Representation Libraries Sequenced with the Illumina Genome Analyzer in Maize
Figure 1
Preparation of paired-end reduced representation libraries. Genomic DNA is digested with methyl-sensitive restriction endonuclease PstI. After random shearing, DNA fragments containing the PstI end are selected via biotin selection and end-sequenced. Resulting sequences are assembled locally to create large contig sequences.