Phylogenetic, Molecular, and Biochemical Characterization of Caffeic Acid o-Methyltransferase Gene Family in Brachypodium distachyon

Figure 4

Tissue-specific expression of BdCOMT genes. Total RNAs were extracted from different Brachypodium tissues as indicated and used for synthesis of cDNA for RT-PCR. Gene-specific primers for each Brachypodium COMT genes were designed and used to amply corresponding transcripts. Primers specific for actin expression are used as the internal control to indicate that equal amount of cDNAs was used in each RT-PCR assay.