Correlation of Vernalization Loci VRN-H1 and VRN-H2 and Growth Habit in Barley Germplasm
Figure 1
(a) Amplification of 437 bp PCR product, by using primers HvBM5.84F and HvBM5.85R, indicative of presence of critical vernalization regulatory region in VRN-H1 locus—a winter type recessive vrn-H1 allele in A: Karoon, B: Shahr-e-Kord, C: Arigashar, D: Bahman, and E: Radical, and no amplification suggestive of a deletion in critical vernalization region in VRN-H1 locus leading to a spring type dominant Vrn-H1 allele in F: Torkman, G: Jonoob, H: Kavir, I: Nosrat, J: Nimrooz, K: Yousof, and L: Morex. (b) Discrimination between winter haplotypes 5C and 1A shown by amplification of 344 bp PCR band, by using primers HvBM5A-intronI-F3b and HvBM5A-intronI-R3b, in A: Karoon, B: Shahr-e-Kord, and C: Arigashar and amplification of 830 bp PCR band by using the same primers in D: Bahman and E: Radical.
(a) Critical vernalization regulatory region in intron I
